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Title: Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors
Author: Liu, Yafeng ; Zhao, Yuan ; Lv, Xiaohua ; Li, Yiding ; Zhang, Xiaohui ; Zhang, Jie ; Wang, Liping ; Zen, Shaoqun
Issued Date: 2012
Conference Name: REVIEW OF SCIENTIFIC INSTRUMENTS
Conference Date: 2012
Conference Place: china
Keyword: 2-PHOTON EXCITATION ; CHANNELRHODOPSIN-2 ; OPTOGENETICS ; LIGHT
Subject: Instruments & Instrumentation ; Physics
Corresponding Author: Zen, SQ(reprint author), Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Peoples R China,sqzeng@mail.hust.edu.cn
English Abstract: Recently developed optogenetics provides a fast, non-invasive, and efficient method for cell activation. However, it is difficult for the optical stimulators used for optogenetics to realize selective multisite fast activation. In this paper, we developed a random-access photostimulator based on a pair of perpendicularly oriented acousto-optic deflectors. Precise laser targeting in the x-y plane was verified, and the lateral spatial resolution of laser intensity after the objective was measured as similar to 1.38 mu m. Photostimulaton of ChETA-expressing astrocytes induced reliable inward currents only if the laser beam was directed onto the targeted cell. In the ChR2-expresing neuron, multiple locations along two dendrites were stimulated, and spatiotemporal integration was observed in the soma with fast multi-site activation. These results demonstrated that this random-access photostimulator would be a powerful tool for selective multi-site fast activation. The compact and modular design of this photostimulator makes it easily integrated with different commercial microscopes, and thus widely popularized in many laboratories. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3689954]
Indexed Type: SCI
Sponsorship: NSFC[30927001, 30925013]
Source: REVIEW OF SCIENTIFIC INSTRUMENTS
Language: 英语
Content Type: 会议论文
URI: http://ir.sibs.ac.cn/handle/331001/1501
Appears in Collections:神经所_会议论文

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