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Title: RhoA-ROCK-Myosin pathway regulates morphological plasticity of cultured olfactory ensheathing cells
Author: Huang, Zhi-hui ; Wang, Ying ; Yuan, Xiao-bing ; He, Cheng
Source: EXPERIMENTAL CELL RESEARCH
Issued Date: 2011
Volume: 317, Issue:20, Pages:2823-2834
Keyword: Olfactory ensheathing cells ; RhoA ; Morphological plasticity ; Regeneration ; Myosin ; SPINAL-CORD ; IN-VITRO ; LYSOPHOSPHATIDIC ACID ; CYTOSKELETAL PROTEINS ; ASTROCYTE STELLATION ; CLINICAL-APPLICATION ; AXONAL REGENERATION ; NEURITE RETRACTION ; NGF-RECEPTOR ; MIGRATION
Subject: Oncology ; Cell Biology
Corresponding Author: Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn ; chenghe@smmu.edu.cn
English Abstract: Olfactory ensheathing cells (OECs) are glial cells in the olfactory system with morphological and functional plasticity. Cultured OECs have the flattened and process-bearing shape. Reversible changes have been found between these two morphological phenotypes. However, the molecular mechanism underlying the regulation of their morphological plasticity remains elusive. Using RhoA FRET biosensor, we found that the active RhoA signal mainly distributed in the lamellipodia and/or filopodia of OECs. Local disruption of these active RhoA distributions led to the morphological change from the flattened into process-bearing shape and promoted process outgrowth. Furthermore, RhoA pathway inhibitors, Toxin-B, C3, Y-27632 or over-expression of DN-RhoA blocked serum-induced morphological change of OECs from the process-bearing into flattened shape, whereas the activation of RhoA pathway by lysophosphatidic acid (LPA) promoted the morphological change from the process-bearing into flattened shape. Finally, ROCK-Myosin-F-actin as a downstream of RhoA pathway was involved in morphological plasticity of OECs. Taken together, these results suggest that RhoA-ROCK-Myosin pathway mediates the morphological plasticity of cultured OECs in response to extracellular cues. (C) 2011 Elsevier Inc. All rights reserved.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1516
Appears in Collections:神经所(总)_期刊论文

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Huang, Zhi-hui; Wang, Ying; Yuan, Xiao-bing; He, Cheng.RhoA-ROCK-Myosin pathway regulates morphological plasticity of cultured olfactory ensheathing cells,EXPERIMENTAL CELL RESEARCH,2011,317(20):2823-2834
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