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Title: Precise Spatiotemporal Control of Optogenetic Activation Using an Acousto-Optic Device
Author: Wang, Kaiyu ; Liu, Yafeng ; Li, Yiding ; Guo, Yanmeng ; Song, Peipei ; Zhang, Xiaohui ; Zeng, Shaoqun ; null(王佐仁)
Source: PLOS ONE
Issued Date: 2011
Volume: 6, Issue:12, Pages:e28468-e28469
Keyword: CAENORHABDITIS-ELEGANS ; OPTICAL INTERROGATION ; 2-PHOTON EXCITATION ; NEURONAL-ACTIVITY ; NEURAL CIRCUITS ; MOUSE-BRAIN ; CHANNELRHODOPSIN-2 ; LIGHT ; MANIPULATION ; CHANNEL
Subject: Life Sciences & Biomedicine - Other Topics
Corresponding Author: Wang, KY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai, Peoples R China,xhzhang@ion.ac.cn ; sqzeng@mail.hust.edu.cn ; zuorenwang@ion.ac.cn
English Abstract: Light activation and inactivation of neurons by optogenetic techniques has emerged as an important tool for studying neural circuit function. To achieve a high resolution, new methods are being developed to selectively manipulate the activity of individual neurons. Here, we report that the combination of an acousto-optic device (AOD) and single-photon laser was used to achieve rapid and precise spatiotemporal control of light stimulation at multiple points in a neural circuit with millisecond time resolution. The performance of this system in activating ChIEF expressed on HEK 293 cells as well as cultured neurons was first evaluated, and the laser stimulation patterns were optimized. Next, the spatiotemporally selective manipulation of multiple neurons was achieved in a precise manner. Finally, we demonstrated the versatility of this high-resolution method in dissecting neural circuits both in the mouse cortical slice and the Drosophila brain in vivo. Taken together, our results show that the combination of AOD-assisted laser stimulation and optogenetic tools provides a flexible solution for manipulating neuronal activity at high efficiency and with high temporal precision.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1518
Appears in Collections:神经所(总)_期刊论文
神经环路与动物行为研究组_期刊论文

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Recommended Citation:
Wang, Kaiyu; Liu, Yafeng; Li, Yiding; Guo, Yanmeng; Song, Peipei; Zhang, Xiaohui; Zeng, Shaoqun; Wang, Zuoren.Precise Spatiotemporal Control of Optogenetic Activation Using an Acousto-Optic Device,PLOS ONE,2011,6(12):e28468-e28469
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