中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: MicroRNAs modulate the Wnt signaling pathway through targeting its inhibitors
Author: Liu, Yueguang ; Huang, Tianwen ; Zhao, Xiaolin ; null(程乐平)
Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Issued Date: 2011
Volume: 408, Issue:2, Pages:259-264
Keyword: MicroRNA ; Inhibitors ; Wnt/beta-catenin pathway ; Gsk3b ; Wnt1-cre ; Dicer (Dicer1) conditional knockout ; NEURAL CREST ; BETA-CATENIN ; INT-1 PROTOONCOGENE ; MIDBRAIN ; MALFORMATION ; PROGENITORS ; FAILURE ; BRAIN ; DICER ; GENE
Subject: Biochemistry & Molecular Biology ; Biophysics
Corresponding Author: Cheng, LP (reprint author), Chinese Acad Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,lpcheng@ion.ac.cn
English Abstract: Increasing evidence indicates that microRNAs (miRNAs) play important roles in mouse brain development. We and several other reports recently have demonstrated that Wnt1-cre-mediated loss of Dicer, the key enzyme for miRNA biosynthesis, results in malformation of the midbrain and cerebellum and failure of neural crest and dopaminergic differentiation. The underlying mechanisms, however, remain poorly understood. The resemblance of some of the phenotypes in the Wnt1-cre Dicer conditional knockout embryos and Wnt1(-/-),Wnt1(-/-);Wnt3(-/-) and Wnt1-cre;beta-cateninn(flox/flox) knockout embryos reminds us that loss of miRNA may disrupt the Wnt-beta-catenin signaling. Here we provide evidence that miRNAs modulate the Wnt signaling pathway through targeting its inhibitors. First, we predicted miRNA binding sites in the 3' UTRs of candidate inhibitors of the Wnt signaling pathway and luciferase assays revealed that several inhibitors of Wnt signaling pathway were targeted by miRNAs. Second, we demonstrated that several miRNAs could modulate the expression of Gsk3b. an inhibitor of Wnt signaling, post-transcriptional in 293T cells. Third, we found that several miRNAs were able to regulate the Wnt-beta-catenin signaling activity in 293T cells. More interestingly, the expression of beta-catenin protein was dramatically reduced in the Wnt1-cre-meidiated Dicer knockout brain tissue compared with control. Our studies therefore suggest that miRNAs might exert their functions, at least in part, by modulating the Wnt signaling pathway through targeting its inhibitors. (C) 2011 Elsevier Inc. All rights reserved.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1538
Appears in Collections:神经所(总)_期刊论文
神经发育及其调控机理研究组_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Liu-2011-MicroRNAs modulate t.pdf(340KB)----开放获取View Download

Recommended Citation:
Liu, Yueguang; Huang, Tianwen; Zhao, Xiaolin; Cheng, Leping.MicroRNAs modulate the Wnt signaling pathway through targeting its inhibitors,BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,408(2):259-264
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Yueguang]'s Articles
[Huang, Tianwen]'s Articles
[Zhao, Xiaolin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Yueguang]‘s Articles
[Huang, Tianwen]‘s Articles
[Zhao, Xiaolin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Liu-2011-MicroRNAs modulate t.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!