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Title: Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth
Author: Yang, Guo-Ying ; Liang, Bin ; Zhu, Ji ; null(罗振革)
Source: JOURNAL OF BIOLOGICAL CHEMISTRY
Issued Date: 2011
Volume: 286, Issue:8, Pages:6566-6576
Keyword: PROTEIN-KINASE-C ; BETA-CATENIN ; NEURONAL POLARIZATION ; PROTEOLYTIC ACTIVITY ; MEMBRANE PROTRUSION ; FRIZZLED HOMOLOGS ; SIGNALING PATHWAY ; MAMMALIAN CORTEX ; CELL-MIGRATION ; CONE FORMATION
Subject: Biochemistry & Molecular Biology
Corresponding Author: Luo, ZG (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zgluo@ion.ac.cn
English Abstract: Axon development involves spatial-temporal cytoskeletal reorganization. However, how the cytoskeleton remodeling is modulated by extracellular cues is unclear. Here, we report a role of Wnt/Ca(2+) signaling in regulating actin and growth cone dynamics. We found that treatment of cultured cortical neurons with Wnt5a, a non-canonical Wnt, either globally or locally, caused an increase in the activity of calpain, a calcium-dependent protease responsible for the cleavage of several actin binding proteins, including spectrin. Treatment with Wnt5a promoted growth cone advance, as well as axonal growth, and these effects were prevented by chelating intracellular calcium, inhibition or down-regulation of calpain, or blockade of spectrin cleavage by competitive peptides. Interestingly, both Wnt5a and activated calpain were found to be mainly distributed in the axon-rich intermediate zone of neocortex. Down-regulating calpain expression interfered with the growth of callosal axons in vivo. Thus, Wnt5a serves as a physiological cue to stimulate localized calpain activity, which in turn promotes growth cone advance and axonal growth.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1551
Appears in Collections:神经所(总)_期刊论文
突触信号研究组_期刊论文

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Recommended Citation:
Yang, Guo-Ying; Liang, Bin; Zhu, Ji; Luo, Zhen-Ge.Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth,JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(8):6566-6576
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