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Title: Extracellular Spermine Exacerbates Ischemic Neuronal Injury through Sensitization of ASIC1a Channels to Extracellular Acidosis
Author: Duan, Bo ; Wang, Yi-Zhi ; Yang, Tao ; Chu, Xiang-Ping ; Yu, Ye ; Huang, Yu ; Cao, Hui ; Hansen, Jillian ; Simon, Roger P. ; Zhu, Michael X. ; Xiong, Zhi-Gang ; Xu, Tian-Le(徐天乐)
Source: JOURNAL OF NEUROSCIENCE
Issued Date: 2011
Volume: 31, Issue:6, Pages:2101-2112
Keyword: SENSING ION-CHANNEL ; CEREBRAL-ISCHEMIA ; HIPPOCAMPAL-NEURONS ; INDUCED NEUROTOXICITY ; POLYAMINE TRANSPORT ; ACTIVATED CURRENTS ; RAT-BRAIN ; CALCIUM ; RECEPTOR ; CONTRIBUTES
Subject: Neurosciences & Neurology
Corresponding Author: Xu, TL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn
English Abstract: Ischemic brain injury is a major problem associated with stroke. It has been increasingly recognized that acid-sensing ion channels (ASICs) contribute significantly to ischemic neuronal damage, but the underlying mechanism has remained elusive. Here, we show that extracellular spermine, one of the endogenous polyamines, exacerbates ischemic neuronal injury through sensitization of ASIC1a channels to extracellular acidosis. Pharmacological blockade of ASIC1a or deletion of the ASIC1 gene greatly reduces the enhancing effect of spermine in ischemic neuronal damage both in cultures of dissociated neurons and in a mouse model of focal ischemia. Mechanistically, spermine profoundly reduces desensitization of ASIC1a by slowing down desensitization in the open state, shifting steady-state desensitization to more acidic pH, and accelerating recovery between repeated periods of acid stimulation. Spermine-mediated potentiation of ASIC1a activity is occluded by PcTX1 (psalmotoxin 1), a specific ASIC1a inhibitor binding to its extracellular domain. Functionally, the enhanced channel activity is accompanied by increased acid-induced neuronal membrane depolarization and cytoplasmic Ca(2+) overload, which may partially explain the exacerbated neuronal damage caused by spermine. More importantly, blocking endogenous spermine synthesis significantly attenuates ischemic brain injury mediated by ASIC1a but not that by NMDA receptors. Thus, extracellular spermine contributes significantly to ischemic neuronal injury through enhancing ASIC1a activity. Our data suggest new neuroprotective strategies for stroke patients via inhibition of polyamine synthesis and subsequent spermine-ASIC interaction.
Indexed Type: SCI
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1553
Appears in Collections:神经所(总)_期刊论文

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Duan, Bo; Wang, Yi-Zhi; Yang, Tao; Chu, Xiang-Ping; Yu, Ye; Huang, Yu; Cao, Hui; Hansen, Jillian; Simon, Roger P.; Zhu, Michael X.; Xiong, Zhi-Gang; Xu, Tian-Le.Extracellular Spermine Exacerbates Ischemic Neuronal Injury through Sensitization of ASIC1a Channels to Extracellular Acidosis,JOURNAL OF NEUROSCIENCE,2011,31(6):2101-2112
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