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Title: Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition
Author: Huang, Zhi-hui ; Wang, Ying ; Su, Zhi-da ; Geng, Jian-guo ; Chen, Yi-zhang ; Yuan, Xiao-bing ; He, Cheng
Source: JOURNAL OF CELL SCIENCE
Issued Date: 2011
Volume: 124, Issue:2, Pages:186-197
Keyword: Ca2+ ; Cofilin ; Migration ; Olfactory ensheathing cells ; RhoA ; Slit-2 ; ACTIN-FILAMENT TURNOVER ; LIM-KINASE ; GROWTH CONE ; NEURONAL MIGRATION ; SLINGSHOT PHOSPHATASE ; AXON GUIDANCE ; NEURITE OUTGROWTH ; FAMILY GTPASES ; SPINAL-CORD ; IN-VITRO
Subject: Cell Biology
Corresponding Author: Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn ; chenghe@smmu.edu.cn
English Abstract: Olfactory ensheathing cells (OECs) migrate from the olfactory epithelium towards the olfactory bulb during development. However, the guidance mechanism for OEC migration remains a mystery. Here we show that migrating OECs expressed the receptor of the repulsive guidance factor Slit-2. A gradient of Slit-2 in front of cultured OECs first caused the collapse of the leading front, then the reversal of cell migration. These Slit-2 effects depended on the Ca2+ release from internal stores through inositol (1,4,5)-triphosphate receptor channels. Interestingly, in response to Slit-2 stimulation, collapse of the leading front required the activation of the F-actin severing protein cofilin in a Ca2+-dependent manner, whereas the subsequent reversal of the soma migration depended on the reversal of RhoA activity across the cell. Finally, the Slit-2-induced repulsion of cell migration was fully mimicked by co-application of inhibitors of F-actin polymerization and RhoA kinase. Our findings revealed Slit-2 as a repulsive guidance factor for OEC migration and an unexpected link between Ca2+ and cofilin signaling during Slit-2-triggered repulsion.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1557
Appears in Collections:神经所(总)_期刊论文

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Huang, Zhi-hui; Wang, Ying; Su, Zhi-da; Geng, Jian-guo; Chen, Yi-zhang; Yuan, Xiao-bing; He, Cheng.Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition,JOURNAL OF CELL SCIENCE,2011,124(2):186-197
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