中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease
Author: Wang, E. -S. ; Sun, Y. ; Guo, J. -G. ; Gao, X. ; Hu, J. -W. ; Zhou, L. ; Hu, J. ; Jiang, C. -C.
Source: ACTA NEUROLOGICA SCANDINAVICA
Issued Date: 2010
Volume: 122, Issue:5, Pages:350-359
Keyword: Parkinson's disease ; cerebrospinal fluid ; biomarker ; proteomics ; DIFFERENCE GEL-ELECTROPHORESIS ; ALZHEIMERS-DISEASE ; MULTIPLE-SCLEROSIS ; PROTEOMIC ANALYSIS ; NEURODEGENERATIVE DISEASES ; PROTEIN MARKERS ; 2-D DIGE ; CSF ; BRAIN ; EXPRESSION
Subject: Neurosciences & Neurology
Corresponding Author: Jiang, CC (reprint author), Fudan Univ, Huashan Hosp, Dept Neurosurg, 12 Wulumuqi Middle Rd, Shanghai 200040, Peoples R China,wesong@sina.com
English Abstract: Objective - The application of biomarkers may potentially improve the efficiency of the diagnosis for Parkinson's disease (PD). However, no reliable biomarker has been identified to date. This study is aimed to identify proteins that might serve as potential biomarkers for PD diagnosis or pathogenesis. Materials and methods - Two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), was used to determine the differentially expressed cerebrospinal fluid (CSF) proteins in PD patients (n = 3) compared with normal controls (n = 3). Selected proteins were further confirmed by Western blotting analysis in the CSF of PD patients (n = 8), Alzheimer's disease (AD) patients (n = 6) and normal control subjects (n = 7). Results - Eight proteins were identified after MS and protein database interrogation. In the CSF of PD patients, the expression levels of one isoform of apolipoprotein A-I (apoA-I), tetranectin, myosin phosphatase target subunit 1 (MYPT1), and two unknown proteins were down-regulated, whereas the expression levels of another apoA-I isoform, proapolipoprotein, and lipoprotein were up-regulated. Western blotting indicates that the expression of tetranectin was reduced in the CSF from PD patients and elevated in AD, while the expression of apoA-I was changed only in the CSF from PD patients. Conclusion - Our preliminary results suggest that tetranectin and apoA-I may serve as potential biomarkers for PD, though further validation is needed.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1567
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
j.1600-0404.2009.01318.x.pdf(440KB)----开放获取View Download

Recommended Citation:
Wang, E. -S.; Sun, Y.; Guo, J. -G.; Gao, X.; Hu, J. -W.; Zhou, L.; Hu, J.; Jiang, C. -C..Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease,ACTA NEUROLOGICA SCANDINAVICA,2010,122(5):350-359
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, E. -S.]'s Articles
[Sun, Y.]'s Articles
[Guo, J. -G.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, E. -S.]‘s Articles
[Sun, Y.]‘s Articles
[Guo, J. -G.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: j.1600-0404.2009.01318.x.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!