中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Wnt1-cre-mediated Conditional Loss of Dicer Results in Malformation of the Midbrain and Cerebellum and Failure of Neural Crest and Dopaminergic Differentiation in Mice
Author: Huang, Tianwen ; Liu, Yueguang ; Huang, Menggui ; Zhao, Xiaolin ; null(程乐平)
Source: JOURNAL OF MOLECULAR CELL BIOLOGY
Issued Date: 2010
Volume: 2, Issue:3, Pages:152-163
Keyword: Wnt1-cre ; Dicer (Dicer1) knockout ; microRNA ; neural crest ; midbrain and hindbrain ; head skeleton ; sympathetic ganglia ; neuropeptide Y ; dopaminergic neuron development ; CENTRAL-NERVOUS-SYSTEM ; ENZYME DICER ; OLIGODENDROCYTE DIFFERENTIATION ; NEUROTRANSMITTER PHENOTYPE ; INT-1 PROTOONCOGENE ; TISSUE ORIGINS ; CELL LINEAGE ; MOUSE-BRAIN ; MORPHOGENESIS ; NEURONS
Subject: Cell Biology
Corresponding Author: Cheng, LP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,lpcheng@ion.ac.cn
English Abstract: The involvement of microRNAs (miRNAs) in the development of the neural crest (NC) cells and other neuronal differentiation is still poorly understood. Here, we investigated the global function of miRNAs in embryonic development by examining the Wnt1-cre-mediated Dicer knockout mice. Dicer ablation resulted in malformation of the midbrain and cerebellum and failure of NC and dopaminergic differentiation. First, the Dicer mutant fetuses exhibited dramatic malformation of the tectum and cerebellum and the eyelids were open. Second, the skeletal structures that are derived from the cranial NC were lost or mostly ablated in Dicer mutant mice. Third, deletion of Dicer in the NC cells resulted in the malformation of the dorsal root ganglia, enteric nervous system and sympathetic ganglia. Interestingly, the expression of neuropeptide Y and its potential regulators TrkA, AP-2 alpha and AP-2 beta was largely abolished in sympathetic neurons of Dicer mutant mice. Fourth, in situ hybridization data revealed that the expression of miR-9, miR-124 and miR-218 in the midbrain and rostral hindbrain area was mostly eliminated in the Dicer mutant mice. We then demonstrated that the development of dopaminergic neurons was impaired in Dicer-deleted mice. Our studies therefore suggest that miRNAs contribute to the embryonic development in multiple locations.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1589
Appears in Collections:神经所(总)_期刊论文
神经发育及其调控机理研究组_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
jmcb.mjq008.full.pdf(721KB)----开放获取View Download

Recommended Citation:
Huang, Tianwen; Liu, Yueguang; Huang, Menggui; Zhao, Xiaolin; Cheng, Leping.Wnt1-cre-mediated Conditional Loss of Dicer Results in Malformation of the Midbrain and Cerebellum and Failure of Neural Crest and Dopaminergic Differentiation in Mice,JOURNAL OF MOLECULAR CELL BIOLOGY,2010,2(3):152-163
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, Tianwen]'s Articles
[Liu, Yueguang]'s Articles
[Huang, Menggui]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, Tianwen]‘s Articles
[Liu, Yueguang]‘s Articles
[Huang, Menggui]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: jmcb.mjq008.full.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!