Background: The objective of this study was to determine the effect of erythropoietin (Epo) on the intestinal labile zinc and the inflammatory factor in rats after traumatic brain injury (TBI). Methods: Male Sprague-Dawley rats were randomly divided into nine groups: (a) normal group; (b) sham-operation group; (c, d, e, f, and g) TBI group, killed at I hour, 6 hour, 24 hour, and 72 hour and 7 days postinjury, respectively; (h and i) TBI + saline and TBI + Epo, killed at 24 hour or 72 hour postinjury. Parietal brain contusion was produced by a free-falling weight on the exposed dura of the right parietal lobe. Intestinal labile zinc, the tumor necrosis factor-a, interleukin (IL)-8, and wet/dry weight ratio were investigated in different groups. Results: The gut contains a certain amount of labile zinc in normal animals and TBI caused obviously gradual increment of intestinal liabled zinc. The levels of inflammatory mediators and the gut wet/dry weight ratio were also found to increase in the trauma group (p < 0.05). There was a highly positive correlation between the abundance of zinc fluorescence and these proinflammation factors. Epo significantly reduced the intestinal labile zinc, the inflammatory mediators, and the gut wet/dry weight ratio compared with TBI group (p < 0.05). Conclusions: Epo can protect intestine from TBI-induced injury by attenuating intestinal inflammation and labile zinc accumulation in vivo.
Zhu, Lin; Jin, Wei; Pan, Hao; Hu, Zelan; Zhou, Jing; Hang, Chunhua; Shi, Jixin.Erythropoietin Inhibits the Increase of Intestinal Labile Zinc and the Expression of Inflammatory Mediators After Traumatic Brain Injury in Rats,JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE,2009,66(3):730-736