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Title: Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures
Author: Luo, Xiaoying ; Zhang, Xinhua ; Shao, Wei ; Yin, Yanqin ; null(周嘉伟)
Source: JOURNAL OF NEUROCHEMISTRY
Issued Date: 2009
Volume: 108, Issue:4, Pages:952-961
Keyword: apomorphine ; astrocyte ; FGF-2 promoter ; gene regulation ; MZF-1 ; GROWTH FACTOR-2 EXPRESSION ; BLOOD-BRAIN-BARRIER ; PARKINSONS-DISEASE ; DOPAMINERGIC-NEURONS ; STRIATAL ASTROCYTES ; CARDIAC MYOCYTES ; PROMOTER REGION ; BASAL GANGLIA ; FACTOR GENE ; FIBROBLAST
Subject: Biochemistry & Molecular Biology ; Neurosciences & Neurology
Corresponding Author: Zhou, JW (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurobiol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China,jwzhou@ion.ac.cn
English Abstract: Apomorphine (APO), a potent D1/D2 dopamine receptor agonist, is used as an anti-parkinsonian drug. It stimulates the synthesis and release of multiple trophic factors in mesencephalic and striatal neurons, preventing the loss of dopaminergic neurons in vitro. Furthermore, APO enhances the biosynthesis and release of FGF-2 by activating dopamine receptors in striatal astrocytes, where cAMP/PKA and PKC/MAPK signalling cascades mediate this process. We investigate the effects of APO on the fibroblast growth factor-2 (FGF-2) promoter and its regulation in astrocytes and identify the transcription factor and cis element underlying these effects. In screening for cis-acting elements over the entire region of the FGF-2 promoter stimulated by APO in the astrocytes, a sequence located in the -785/-745 region was found to serve as the cis element. This element was recognized by the human myeloid zinc finger protein 1 (MZF-1) transcription factor. Introducing human MZF-1 plasmid and human MZF-1-specific siRNA has different effects on the FGF-2 promoter. Furthermore, it increases FGF-2 protein expression in HeLa cells and primary astrocytes, indicating that APO stimulates the FGF-2 promoter via the MZF-1 transcription factor. These data suggest that APO can enhance the biosynthesis and release of FGF-2 through the activation of the MZF-1 transcription factor in striatal astrocytes.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1681
Appears in Collections:神经所(总)_期刊论文
基底神经节的发育与退行性疾病研究组_期刊论文

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Luo, Xiaoying; Zhang, Xinhua; Shao, Wei; Yin, Yanqin; Zhou, Jiawei.Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures,JOURNAL OF NEUROCHEMISTRY,2009,108(4):952-961
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