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Title: Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells
Author: Wang, Hai-Bo ; Guan, Ji-Song ; Bao, Lan ; null(张 旭)
Source: NEUROCHEMICAL RESEARCH
Issued Date: 2008
Volume: 33, Issue:10, Pages:2028-2034
Keyword: delta-Opioid receptor ; tag-fused receptor ; large dense-core vesicle ; subcellular distribution ; PLASMA-MEMBRANE ; GLYCINE RECEPTOR ; PICHIA-PASTORIS ; SPINAL-CORD ; PROTEIN ; EXPRESSION ; TRAFFICKING ; AGONIST ; RAT ; LOCALIZATION
Subject: Biochemistry & Molecular Biology ; Neurosciences & Neurology
Corresponding Author: Zhang, X (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,xu.zhang@ion.ac.cn
English Abstract: In small dorsal root ganglion neurons, delta-opioid receptors (DORs) have been found to be mainly distributed in the cytoplasm and often associated with the membrane of large dense-core vesicles (LDCVs) that contain neuropeptides. To study the distribution of DORs under various physiological or pharmacological conditions, the receptors fused with different tags are constructed, transfected into cells or animals, and examined with microscopy. In this study, we show that DOR with different tags have distinct patterns of subcellular distribution in neuroendocrine cells, PC12 cells. Both immunostaining and vesicle fraction analysis showed that the native DORs expressed in PC12 cells were mainly associated with LDCVs. In transfected PC12 cells, DOR tagged with Myc or hemagglutinin exhibited LDCV localization. However, DOR fused with GFP at N- or C-terminus was found to be mainly localized on the cell surface, and mediated the function of DOR agonist. Therefore, the distribution of DOR fused with GFP differs from the native DORs. These results suggest that the subcellular distribution of the receptor could be better presented by the fused tag with smaller molecular size.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1699
Appears in Collections:神经所(总)_期刊论文
感觉系统研究组_期刊论文

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Wang, Hai-Bo; Guan, Ji-Song; Bao, Lan; Zhang, Xu.Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells,NEUROCHEMICAL RESEARCH,2008,33(10):2028-2034
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