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Title: Clathrin-dependent endocytosis is required for TrkB-dependent Akt-mediated neuronal protection and dendritic growth
Author: Zheng, Jing ; Shen, Wan-Hua ; Lu, Ting-Jia ; Zhou, Yang ; Chen, Qian ; Wang, Zi ; Xiang, Ting ; Zhu, Yong-Chuan ; Zhang, Chi ; Duan, Shumin ; null(熊志奇)
Source: JOURNAL OF BIOLOGICAL CHEMISTRY
Issued Date: 2008
Volume: 283, Issue:19, Pages:13280-13288
Keyword: NEUROTROPHIC FACTOR ; SIGNALING PATHWAYS ; RECEPTOR INTERNALIZATION ; IN-VITRO ; SURVIVAL ; EXPRESSION ; KINASE ; CELLS ; BDNF ; NGF
Subject: Biochemistry & Molecular Biology
Corresponding Author: Xiong, ZQ (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China,xiongzhiqi@ion.ac.cn
English Abstract: Endocytosis of Trk ((t) under bar ropomyosin-(r) under bar elated (k) under bar inase) receptors is critical for neurotrophin signal transduction and biological functions. However, the mechanism governing endocytosis of TrkB ((t) under bar ropomyosin-(r) under bar elated (k) under bar inase (B) under bar) and the specific contributions of TrkB endocytosis to downstream signaling are unknown. In this study, we report that blocking clathrin, dynamin, or AP2 in cultured neurons of the central nervous system inhibited brain-derived neurotrophic factor (BDNF)-induced activation of Akt but not ERK. Treating neurons with the clathrin inhibitor monodansylcadaverine or a peptide that blocks dynamin function specifically abrogated Akt pathway activation in response to BDNF but did not affect the response of other downstream effectors or the up-regulation of immediate early genes neuropeptide Y and activity-regulated cytoskeleton-associated protein. Similar effects were found in neurons expressing small interfering RNA to silence AP2 or a dominant negative form of dynamin that inhibits clathrin-mediated endocytosis. In PC12 cells, ERK but not Akt activation required TrkA endocytosis following stimulation with nerve growth factor, whereas the opposite was true when TrkA-expressing neurons were stimulated with nerve growth factor in the central nervous system. Thus, the specific effects of internalized Trk receptors probably depend on the presence of cell type-specific modulators of neurotrophin signaling and not on differences inherent to Trk receptors themselves. Endocytosis-dependent activation of Akt in neurons was found to be critical for BDNF-supported survival and dendrite outgrowth. Together, these results demonstrate the functional requirement of clathrin-and dynamin-dependent endocytosis in generating the full intracellular response of neurons to BDNF in the central nervous system.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1729
Appears in Collections:神经所(总)_期刊论文
疾病神经生物学研究组_期刊论文

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Zheng, Jing; Shen, Wan-Hua; Lu, Ting-Jia; Zhou, Yang; Chen, Qian; Wang, Zi; Xiang, Ting; Zhu, Yong-Chuan; Zhang, Chi; Duan, Shumin; Xiong, Zhi-Qi.Clathrin-dependent endocytosis is required for TrkB-dependent Akt-mediated neuronal protection and dendritic growth,JOURNAL OF BIOLOGICAL CHEMISTRY,2008,283(19):13280-13288
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