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Title: Critical role of type 2 ryanodine receptor in mediating activity-dependent neurogenesis from embryonic stem cells
Author: Yu, Hui-Mei ; Wen, Jing ; Wang, Rong ; Shen, Wan-Hua ; Duan, Shumin ; Yang, Huang-Tian
Source: CELL CALCIUM
Issued Date: 2008
Volume: 43, Issue:5, Pages:417-431
Keyword: neurogenesis ; ryanodine receptors ; ES cells ; L-type Ca2+ channels ; GABA(A) receptors ; HIPPOCAMPAL PROGENITOR CELLS ; CALCIUM-RELEASE ; CA2+ CHANNELS ; NEURONAL DIFFERENTIATION ; ENDOPLASMIC-RETICULUM ; CARDIAC MYOCYTES ; SKELETAL-MUSCLE ; IN-VITRO ; GABA ; PROLIFERATION
Subject: Cell Biology
Corresponding Author: Yang, HT (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China,htyang@sibs.ac.cn
English Abstract: Activity-induced neurogenesis via Ca2+ entry may be important for establishing Hebbian neural network. However, it remains unclear whether intracellular Ca2+ mobilization is required and which subtypes of Ca2+ release channels expressed in Ca2+ store organelles are involved in the activity-dependent neurogenesis. Here, we demonstrated that the activity of intracellular Ca2+ signaling, expression of neuronal transcription factor NeuroD, and the rate of neurogenesis were significantly inhibited in neuronal cells derived from embryonic stem (ES) cells deficient in the Ca2+ release channel type 2 ryanodine receptors (RyR2(-/-)). In wild-type (RyR2(+/+)) but not in RyR2(-/-) ES cells, activation of L-type Ca2+ channels, GABA(A) receptors, or RyRs promoted neuronal differentiation, while inhibition of these channels/receptors had an opposite effect. Moreover, neuronal differentiation promoted by activation of GABA(A) receptors or L-type Ca2+ channels in RyR2(+/+) cells was prevented by RyR inhibitors. No significant difference was detected in the expression level of GABA(A) receptors and L-type channels between neuronal cells derived from two types of ES cells. Thus, activity-induced Ca2+ influx through L-type Ca2+ channels alone is not sufficient in promoting neurogenesis. Instead, an intimate cooperation of L-type Ca2+ channels with RyR2 is crucial for the activity-dependent neurogenesis induced by paracrine and/or autocrine GABA signaling. (c) 2007 Elsevier Ltd. All rights reserved.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1731
Appears in Collections:神经所(总)_期刊论文

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Yu, Hui-Mei; Wen, Jing; Wang, Rong; Shen, Wan-Hua; Duan, Shumin; Yang, Huang-Tian.Critical role of type 2 ryanodine receptor in mediating activity-dependent neurogenesis from embryonic stem cells,CELL CALCIUM,2008,43(5):417-431
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