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Title: A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons
Author: Zhang, Wei ; Ge, Wooping ; null(王佐仁)
Source: EUROPEAN JOURNAL OF NEUROSCIENCE
Issued Date: 2007
Volume: 26, Issue:9, Pages:2405-2416
Keyword: dopaminergic neuron ; giant ; gustatory neuron ; nociception ; painless ; MUSHROOM BODY ; SYNAPTIC-TRANSMISSION ; TASTE PERCEPTION ; ADULT DROSOPHILA ; EXCITABLE CELLS ; OPTICAL CONTROL ; MELANOGASTER ; ACTIVATION ; FIBER ; GENE
Subject: Neurosciences & Neurology
Corresponding Author: Zhang, W (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China,ZuorenWang@ion.ac.cn
English Abstract: In order to study the function of specific neural circuits, we generated UAS-Channelrhodopsin2 (ChR2) transgenic Drosophila and established a ChR2-based system that enables specific activation of targeted neurons in larval and adult fruit flies with blue light illumination, under the control of a newly designed light source that provides fully programmable stimulation patterns. We showed that stimulating selectively the nociceptor of larvae expressing ChR2 elicited light-induced 'pain' response, confined freely behaving larvae in defined area and directed larva migration along a preset route. In freely behaving adult flies, rapid photoactivation of targeted gustatory sensory neurons, dopaminergic modulatory neurons and motor neurons triggered the proboscis extension response, escaping reflex and changes in the locomotion pattern, respectively, with precise temporal control. This non-invasive method for remote control of animal behaviors also provides a potential tool for conducting 'gain of function' studies toward understanding how animal behaviors are controlled by neural activity.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1749
Appears in Collections:神经所(总)_期刊论文
神经环路与动物行为研究组_期刊论文

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Zhang, Wei; Ge, Wooping; Wang, Zuoren.A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons,EUROPEAN JOURNAL OF NEUROSCIENCE,2007,26(9):2405-2416
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