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Title: Regulated ATP release from astrocytes through lysosome exocytosis
Author: Zhang, Zhijun ; Chen, Gang ; Zhou, Wei ; Song, Aihong ; Xu, Tao ; Luo, Qingming ; null(王 伟) ; Gu, Xiao-song ; Duan, Shumin
Source: NATURE CELL BIOLOGY
Issued Date: 2007
Volume: 9, Issue:8, Pages:945-U115
Keyword: KISS-AND-RUN ; HIPPOCAMPAL SYNAPSES ; MULTIDRUG-RESISTANCE ; SECRETORY LYSOSOMES ; CALCIUM WAVES ; CELLS ; VESICLES ; RECEPTORS ; RELEVANCE ; PROTEINS
Subject: Cell Biology
Corresponding Author: Duan, SM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China,shumin@ion.ac.cn
English Abstract: Release of ATP from astrocytes is required for Ca2+ wave propagation among astrocytes(1-3) and for feedback modulation of synaptic functions(2,4,5). However, the mechanism of ATP release and the source of ATP in astrocytes are still not known. Here we show that incubation of astrocytes with FM dyes leads to selective labelling of lysosomes. Time-lapse confocal imaging of FM dye-labelled fluorescent puncta, together with extracellular quenching and total-internal-reflection fluorescence microscopy (TIRFM), demonstrated directly that extracellular ATP or glutamate induced partial exocytosis of lysosomes, whereas an ischaemic insult with potassium cyanide induced both partial and full exocytosis of these organelles. We found that lysosomes contain abundant ATP, which could be released in a stimulus-dependent manner. Selective lysis of lysosomes abolished both ATP release and Ca2+ wave propagation among astrocytes, implicating physiological and pathological functions of regulated lysosome exocytosis in these cells.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1757
Appears in Collections:神经所(总)_期刊论文
视知觉机制研究组_期刊论文

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Zhang, Zhijun; Chen, Gang; Zhou, Wei; Song, Aihong; Xu, Tao; Luo, Qingming; Wang, Wei; Gu, Xiao-song; Duan, Shumin.Regulated ATP release from astrocytes through lysosome exocytosis,NATURE CELL BIOLOGY,2007,9(8):945-U115
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