中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2
Author: Guo, L. ; Wang, Y.
Source: NEUROSCIENCE
Issued Date: 2007
Volume: 145, Issue:1, Pages:100-109
Keyword: GRIP1 ; degradation ; glutamate ; AMPA receptor ; UPS ; GluR2 ; AMPA RECEPTOR ; SYNAPTIC PLASTICITY ; DIFFERENTIAL PALMITOYLATION ; EXCITATORY SYNAPSES ; TRAFFICKING ; BINDING ; PSD-95 ; GRIP1 ; ENDOCYTOSIS ; SUBUNIT
Subject: Neurosciences & Neurology
Corresponding Author: Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Lab Neural Signal Transduct, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,yzwang@ion.ac.cn
English Abstract: The glutamate receptor interacting protein 1 (GRIP1) is a scaffolding protein in postsynaptic density (PSD), tethering AMPA receptors to other signaling proteins. Here we report that glutamate stimulation caused a rapid reduction in protein levels of GRIP1, but not that of glutamate receptor (GluR) 1, GluR2 and protein interacting with C kinase 1 (PICK1) in rat primary cortical neuron cultures. Down-regulation of GRIP1 by glutamate was blocked by carbobenzoxyl-leucinyl-leucinyl-leucinal (MG132), a proteasome inhibitor and by expression of K48R-ubiquitin, a dominant negative form of ubiquitin. The GRIP1 reduction was inhibited by MK-801, an N-methyl-D-aspartate (NMDA) receptor antagonist, but not by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an AMPA receptor antagonist. EGTA and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetra acetic acid tetrakis (BAPTA), two Ca2+ chelators, but not nifedipine, an L-type Ca2+ channel blocker, prevented GRIP1 degradation. Furthermore, MG132 prevented glutamate-stimulated reduction in surface amount of GluR2, and knockdown of GRIP1 by RNAi against GRIP1 reduced surface GluR2 in neurons. Our results suggest that glutamate induces GRIP1 degradation by proteasome through an NMDA receptor-Ca2+ pathway and that GRIP1 degradation may play an important role in regulating GluR2 surface expression. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1783
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Guo-2007-Glutamate stimulates.pdf(1570KB)----开放获取View Download

Recommended Citation:
Guo, L.; Wang, Y..Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2,NEUROSCIENCE,2007,145(1):100-109
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Guo, L.]'s Articles
[Wang, Y.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Guo, L.]‘s Articles
[Wang, Y.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Guo-2007-Glutamate stimulates.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!