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Title: The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron
Author: Wang, LC ; Xiong, W ; Zheng, J ; Zhou, Y ; Zheng, H ; Zhang, C ; Zheng, LH ; Zhu, XL ; Xiong, ZQ ; Wang, LY ; Cheng, HP ; Zhou, Z(周专)
Source: BIOPHYSICAL JOURNAL
Issued Date: 2006
Volume: 90, Issue:10, Pages:3590-3598
Keyword: ROOT GANGLION NEURONS ; ADRENAL CHROMAFFIN CELLS ; CLATHRIN-MEDIATED ENDOCYTOSIS ; FROG NEUROMUSCULAR JUNCTION ; TRANSMITTER RELEASE ; RAPID ENDOCYTOSIS ; NERVE-TERMINALS ; CENTRAL SYNAPSE ; CALCIUM INFLUX ; RAT ADIPOCYTES
Subject: Biophysics
Corresponding Author: Zhou, Z (reprint author), Peking Univ, Inst Mol Med, 5 Yiheyuan Rd, Beijing 100871, Peoples R China,zzhou@pku.edu.cn
English Abstract: Endocytosis is a fundamental cellular event in membrane retrieval after exocytosis and in the regulation of receptor-mediated signal transduction. In contrast to the well-studied depolarization-induced membrane recycling, little is known about the kinetics of ligand-induced endocytosis of G-protein-coupled receptors in neurons. Here we investigated the kinetics of ligand-receptor binding-induced endocytosis in rat sensory neurons using a membrane capacitance assay. The time constant of ADP-induced endocytosis of P2Y-receptors was determined as 1.7 s. The ADP-induced endocytosis was blocked by antagonists against P2Y, phosphorylation, and clathrin. However, block of dynamin was without effect. The ADP-induced endocytosis was confirmed independently by a single vesicle image technique using a styryl FM2-10. Finally, the receptors were internalized in response to ADP, as determined by GFP-labeled P2Y. We conclude that ligand-receptor binding leads to rapid endocytosis in the cytoplasm of rat dorsal root ganglion neurons.
Indexed Type: SCI
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1826
Appears in Collections:神经所(总)_期刊论文

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Wang, LC; Xiong, W; Zheng, J; Zhou, Y; Zheng, H; Zhang, C; Zheng, LH; Zhu, XL; Xiong, ZQ; Wang, LY; Cheng, HP; Zhou, Z.The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron,BIOPHYSICAL JOURNAL,2006,90(10):3590-3598
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