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Title: Generation and characterization of the PKC gamma-Cre mouse line
Author: Ding, YQ ; Xiang, CX ; Chen, ZF
Source: GENESIS
Issued Date: 2005
Volume: 43, Issue:1, Pages:28-33
Keyword: PKC gamma ; gene targeting ; Cre ; brain ; spinal cord ; PROTEIN-KINASE-C ; PERSISTENT PAIN ; SPINAL-CORD ; EXPRESSION ; MICE ; RESPONSES
Subject: Developmental Biology ; Genetics & Heredity
Corresponding Author: Chen, ZF (reprint author), Washington Univ, Pain Ctr, Sch Med, Dept Anesthesiol, St Louis, MO 63110 USA,chenz@wustl.edu
English Abstract: PKC gamma is a protein kinase C (PKC) isoform that is expressed in the central nervous system (CNS). We generated a PKC gamma-Cre mouse line and characterized the expression and activity of the Cre protein. Our studies show that Cre expression largely recapitulates the endogenous expression of PKC gamma. Several major sites of Cre expression are the cerebral cortex, hippocampus, thalamus, amygdala, and spinal cord. Examination of PKC gamma-Cre/ROSA26 mice reveals a similar X-gal staining pattern in the CNS, indicating that Cre recombinase is capable of removing LoxP sites in vivo. These data indicate that the PKC gamma-Cre mouse line could be a useful reagent for generating Cre-mediated tissue-specific knockouts in the CNS.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1883
Appears in Collections:神经所(总)_期刊论文

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Ding, YQ; Xiang, CX; Chen, ZF.Generation and characterization of the PKC gamma-Cre mouse line,GENESIS,2005,43(1):28-33
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