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Title: Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1
Author: Tao, YM ; Zeng, R ; Shen, B ; Jia, JM ; Wang, YZ
Source: JOURNAL OF NEUROCHEMISTRY
Issued Date: 2005
Volume: 94, Issue:6, Pages:1512-1522
Keyword: neurotransmission ; N-methyl-D-aspartate receptor ; phosphorylation ; protein kinase A ; Ser229 ; voltage-gated K+ channel 1.4 ; GATED POTASSIUM CHANNELS ; RAT HIPPOCAMPAL-NEURONS ; CANNABINOID RECEPTOR MODULATION ; METHYL-D-ASPARTATE ; K+ CHANNELS ; SYNAPTIC PLASTICITY ; STRIATAL NEURONS ; ALPHA-SUBUNITS ; IMMUNOHISTOCHEMICAL LOCALIZATION ; PHOSPHATIDYLINOSITOL 3-KINASE
Subject: Biochemistry & Molecular Biology ; Neurosciences & Neurology
Corresponding Author: Wang, YZ (reprint author), Chinese Acad Sci, Inst Neurosci, SIBS, Grad Sch, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,yzwang@ion.ac.cn
English Abstract: Phosphorylation of voltage- gated K+ channels (Kv) is involved in regulation of neuronal excitability, synaptic plasticity and neuronal survival. Among Kv channels expressed in the CNS, Kv1.4 is located in the soma, dendrite and axon terminus of neurones in most regions of the brain. Here, we show that Ser229 found within the highly conserved T1 domain of Kv1.4 in cultured rat cortical neurones is phosphorylated by protein kinase A (PKA), as demonstrated by in vitro protein kinase assay and Western blotting with a polyclonal antibody specific against phosphorylated Ser229. Glutamate, high concentrations of K+ or K+ channel blockers known to increase neurotransmission all stimulated the phosphorylation of Kv1.4 at Ser229 via N-methyl-D-aspartate (NMDA), but not alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA) receptor, whereas tetradotoxin (TTX), known to block neuronal transmission, and depletion of extracellular Ca2+ inhibited phosphorylation induced by tetraethylammonium ( TEA), a nonselective K+ channel blocker. Mutation of Ser229 to Ala229 enhanced the current density. Taken together, elevation of the neuronal transmission stimulates the phosphorylation of Kv1.4 at Ser229 via the Ca2+ influx through NMDA receptor. Thus, it is possible that neuronal transmission regulates neuronal excitability partially through the phosphorylation of Kv1.4S229.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1885
Appears in Collections:神经所(总)_期刊论文

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Tao, YM; Zeng, R; Shen, B; Jia, JM; Wang, YZ.Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1,JOURNAL OF NEUROCHEMISTRY,2005,94(6):1512-1522
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