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Title: Divalent cation modulation of a-type potassium channels in acutely dissociated central neurons from wide-type and mutant Drosophila
Author: Xu, TX ; Gong, N ; Xu, TL(徐天乐)
Source: JOURNAL OF NEUROGENETICS
Issued Date: 2005
Volume: 19, Issue:2, Pages:87-107
Keyword: I-A ; A-type K+ channels ; Cd2+ ; Zn2+ ; Ca2+ ; and Mg2+ ions ; slo(1) ; Sh(M) mutations ; larval neurons ; whole-cell patch-clamp ; TRANSIENT OUTWARD CURRENT ; NEGATIVE SURFACE-CHARGE ; VENTRICULAR MYOCYTES ; K+ CHANNELS ; ZINC MODULATION ; BINDING-SITE ; RAT ; CALCIUM ; CURRENTS ; SHAKER
Subject: Genetics & Heredity ; Neurosciences & Neurology
Corresponding Author: Xu, TL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Key Lab Neurobiol, 320 Yueyang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn
English Abstract: Drosophila mutants provide an ideal model to study channel-type specificity of ion channel regulation in situ. In this study, the effects of divalent cations on voltage-gated K+ currents were investigated in acutely dissociated central neurons of Drosophila third instar larvae using the whole-cell patch-clamp recording. Our data showed that micromolar Cd2+ enhanced the peak inactivating current (I-A) without affecting the delayed component (I-K ). The same results were obtained in Ca2+-free external solution, and from slo(1) mutation, which eliminates transient Ca2+-activated K+ current. Micromolar d +and Zn, and millimolar Ca and Mg all shifted the steady-state inactivation curve Of IA without affecting the voltage-dependence of I-A activation, whereas millimolar Cd2+ markedly affected both the activation and steady-state inactivation curves for IA. Divalent cations affected IA with different potency; the sequence was: Zn2+ > Cd2+ > Ca2+ > Mg2+. The modulation of I-A by Cd2+ was partially inhibited in Sh(M), a null Shaker (one of I-A-encoding genes) mutation. Taken together, the channel-type specificity, the asymmetric effects on IA activation and inactivation kinetics, and the diverse potency of divalent cations all strongly support the idea that physiological divalent cations modulate A-type K+ channels through specific binding to extracellular sites of the channels.
Indexed Type: SCI
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1913
Appears in Collections:神经所(总)_期刊论文

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Xu, TX; Gong, N; Xu, TL.Divalent cation modulation of a-type potassium channels in acutely dissociated central neurons from wide-type and mutant Drosophila,JOURNAL OF NEUROGENETICS,2005,19(2):87-107
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