中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord
Author: Wu, LJ ; Duan, B ; Mei, YD ; Gao, J ; Chen, JG ; Zhuo, M ; Xu, L ; Wu, MA ; Xu, TL(徐天乐)
Source: JOURNAL OF BIOLOGICAL CHEMISTRY
Issued Date: 2004
Volume: 279, Issue:42, Pages:43716-43724
Keyword: GATED NA+ CHANNEL ; MUTATIONS CAUSING NEURODEGENERATION ; MAMMALIAN DEGENERIN MDEG ; ROOT GANGLION-CELLS ; CAENORHABDITIS-ELEGANS ; HIPPOCAMPAL-NEURONS ; ACTIVATED CURRENTS ; PAIN ; H+ ; EXPRESSION
Subject: Biochemistry & Molecular Biology
Corresponding Author: Xu, TL (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn
English Abstract: Acid-sensing ion channels (ASICs) are ligand-gated cation channels activated by extracellular protons. In periphery, they contribute to sensory transmission, including that of nociception and pain. Here we characterized ASIC-like currents in dorsal horn neurons of the rat spinal cord and their functional modulation in pathological conditions. Reverse transcriptase-nested PCR and Western blotting showed that three ASIC isoforms, ASIC1a, ASIC2a, and ASIC2b, are expressed at a high level in dorsal horn neurons. Electrophysiological and pharmacological properties of the proton-gated currents suggest that homomeric ASIC1a and/or heteromeric ASIC1a + 2b channels are responsible for the proton-induced currents in the majority of dorsal horn neurons. Acidification-induced action potentials in these neurons were compatible in a pH-dependent manner with the pH dependence of ASIC-like current. Furthermore, peripheral complete Freund's adjuvant-induced inflammation resulted in increased expression of both ASIC1a and ASIC2a in dorsal horn. These results support the idea that the ASICs of dorsal horn neurons participate in central sensory transmission/modulation under physiological conditions and may play important roles in inflammation-related persistent pain.
Indexed Type: SCI
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1940
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
J. Biol. Chem.-2004-Wu-43716-24.pdf(352KB)----开放获取View Download

Recommended Citation:
Wu, LJ; Duan, B; Mei, YD; Gao, J; Chen, JG; Zhuo, M; Xu, L; Wu, MA; Xu, TL.Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord,JOURNAL OF BIOLOGICAL CHEMISTRY,2004,279(42):43716-43724
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wu, LJ]'s Articles
[Duan, B]'s Articles
[Mei, YD]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wu, LJ]‘s Articles
[Duan, B]‘s Articles
[Mei, YD]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: J. Biol. Chem.-2004-Wu-43716-24.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!