中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel
Author: Xu, CQ ; He, LL ; Brone, B ; Martin-Eauclaire, MF ; Van Kerkhove, E ; Zhou, Z ; Chi, CW
Source: TOXICON
Issued Date: 2004
Volume: 43, Issue:8, Pages:961-971
Keyword: SK channel ; amino acid sequence ; gene cloning ; phylogenetic tree ; ADRENAL CHROMAFFIN CELLS ; BUTHUS-MARTENSI KARSCH ; UNPAIRED MEDIAN NEURONS ; POTASSIUM CHANNELS ; GATING MODIFIER ; LEIUROTOXIN-I ; ION CHANNELS ; CALCIUM ; VENOM ; APAMIN
Subject: Pharmacology & Pharmacy ; Toxicology
Corresponding Author: Chi, CW (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,chi@sunm.shcnc.ac.cn
English Abstract: Small conductance calcium activated potassium channels (SK) are crucial in the regulation of cell firing frequency in the nervous system and other tissues. In the present work, a novel SK channel blocker, designated BmSKTx1, was purified from the scorpion Buthus martensi Karsh venom. The sequence of the N-terminal 22 amino acid residues was determined by Edman degradation. Using this sequence information, the full-length cDNA and genomic gene of BmSKTx1 were cloned and sequenced. By these analyses, BmSKTx1 was found to be a peptide composed of 31 amino acid residues with three disulfide bonds. It shared little sequence homology with other known scorpion alpha-KTxs but showed close relationship with SK channel blockers in the phylogenetic tree. According to the previous nomenclature, BmSKTx1 was classified as alpha-KTx14.1. We examined the effects of BmSKTx1 on different ion channels of rat adrenal chromaffin cells (RACC) and locust dorsal unpaired median (DUM) neurons. BmSKTx1 selectively inhibited apamin-sensitive SK currents in RACC with K-d of 0.72 p,M and Hill coefficient of 2.2. And it had no effect on Na+, Ca2+, Kv, and BK currents in DUM neuron, indicating that BmSKTx1 was a selective SK toxin. (C) 2004 Elsevier Ltd. All rights reserved.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1957
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Xu-2004-A novel scorpion tox.pdf(479KB)----开放获取View Download

Recommended Citation:
Xu, CQ; He, LL; Brone, B; Martin-Eauclaire, MF; Van Kerkhove, E; Zhou, Z; Chi, CW.A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel,TOXICON,2004,43(8):961-971
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xu, CQ]'s Articles
[He, LL]'s Articles
[Brone, B]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xu, CQ]‘s Articles
[He, LL]‘s Articles
[Brone, B]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Xu-2004-A novel scorpion tox.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!