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Title: Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion
Author: Yu, X ; Duan, KL ; Shang, CF ; Yu, HG ; Zhou, Z
Source: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Issued Date: 2004
Volume: 101, Issue:4, Pages:1051-1056
Keyword: ADRENAL CHROMAFFIN CELLS ; NUCLEOTIDE-GATED CHANNELS ; LONG-TERM POTENTIATION ; RECEPTOR CHANNELS ; PACEMAKER CHANNELS ; SYNAPTIC-TRANSMISSION ; VENTRICULAR MYOCYTES ; FACILITATION ; PERMEATION ; EXPRESSION
Subject: Science & Technology - Other Topics
Corresponding Author: Zhou, Z (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zzhou@ion.ac.cn
English Abstract: The hyperpolarization-activated cation channels (I-h) play a distinct role in rhythmic activities in a variety of tissues, including neurons and cardiac cells. in the present study, we investigated whether Ca2+ can permeate through the hyperpolarization-activated pacemaker channels (HCN) expressed in HEK293 cells and I-h channels in dorsal root ganglion (DRG) neurons. Using combined measurements of whole-cell currents and fura-2 Ca2+ imaging, we found that there is a Ca2(+) influx in proportion to I-h induced by hyperpolarization in HEK293 cells. The I-h channel blockers Cs+ and ZD7288 inhibit both HCN current and Ca2(+) influx. Measurements of the fractional Ca2+ current showed that it constitutes 0.60+/-0.02% of the net inward current through HCN4 at -120 mV. This fractional current is similar to that of the low Ca2+-permeable AMPA-R (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) channels in Purkinje neurons. In DRG neurons, activation of I-h for 30 s also resulted in a Ca2+ influx and an elevated action potential-induced secretion, as assayed by the increase in membrane capacitance. These results suggest a functional significance for I-h channels in modulating neuronal secretion by permitting Ca2+ influx at negative membrane potentials.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1978
Appears in Collections:神经所(总)_期刊论文

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Yu, X; Duan, KL; Shang, CF; Yu, HG; Zhou, Z.Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion,PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2004,101(4):1051-1056
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