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Title: Localization of dopamine D1-receptor to A-type horizontal cells in the rabbit retina by single cell RT-PCR
Author: Li, H ; Gaughwin, P ; Li, N ; He, SG
Source: NEUROSCIENCE LETTERS
Issued Date: 2004
Volume: 355, Issue:40910, Pages:146-148
Keyword: dissociated retinal neurons ; nested PCR ; sequencing ; MAMMALIAN RETINA ; ENDOGENOUS DOPAMINE ; AMACRINE CELLS ; LIGHT ; MODULATION ; RELEASE ; ACID
Subject: Neurosciences & Neurology
Corresponding Author: He, SG (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,s.he@ion.ac.cn
English Abstract: Dopamine is an important neurotransmitter regulating light-dark adaptation in the retina. The effect of dopamine is widespread and dependent on the localization of its receptors. We performed single cell RT-PCR on A-type horizontal cells visually selected from dissociated rabbit retinal neurons. Dopamine D1-receptor mRNA was positively identified. Subsequent sequencing of the fragment showed 82% homology with rat D1 receptor and 87% homology with human D1 receptor. This study supported previous observations that dopamine regulated A-type horizontal cell coupling via D1 receptors at the level of transcription. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/1980
Appears in Collections:神经所(总)_期刊论文

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Li, H; Gaughwin, P; Li, N; He, SG.Localization of dopamine D1-receptor to A-type horizontal cells in the rabbit retina by single cell RT-PCR,NEUROSCIENCE LETTERS,2004,355(40910):146-148
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