中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Asymmetric cross-inhibition between GABA(A) and glycine receptors in rat spinal dorsal horn neurons
Author: Li, Y ; Wu, LJ ; Legendre, P ; Xu, TL(徐天乐)
Source: JOURNAL OF BIOLOGICAL CHEMISTRY
Issued Date: 2003
Volume: 278, Issue:40, Pages:38637-38645
Keyword: BRAIN-STEM MOTONEURONS ; HIPPOCAMPAL-NEURONS ; GUINEA-PIG ; IONOTROPIC RECEPTORS ; COMMISSURAL NEURONS ; CORD NEURONS ; CALCINEURIN ; RESPONSES ; SYNAPSES ; CHANNELS
Subject: Biochemistry & Molecular Biology
Corresponding Author: Xu, TL (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,
English Abstract: Presynaptic nerve terminals of inhibitory synapses in the dorsal horn of the spinal cord and brain stem can release both GABA and glycine, leading to coactivation of postsynaptic GABA(A) and glycine receptors. In the present study we have analyzed functional interactions between GABA(A) and glycine receptors in acutely dissociated neurons from rat sacral dorsal commissural nucleus. Although the application of GABA and glycine activates pharmacologically distinct receptors, the current induced by a simultaneous application of these two transmitters was less than the sum of currents induced by applying two transmitters separately. Sequential application of glycine and GABA revealed that the GABA-evoked current is more affected by glycine than glycine-evoked responses by GABA. Activation of glycine receptors decreased the amplitude and accelerated the rate of desensitization of GABA-induced currents. This asymmetric cross-inhibition is reversible, dependent on the agonist concentration applied, but independent of both membrane potential and intracellular calcium concentration or changes in the chloride equilibrium potential. During sequential applications, the asymmetric cross-inhibition was prevented by selective GABA(A) or glycine receptor antagonists, suggesting that occupation of binding sites did not suffice to induce glycine and GABA(A) receptors functional interaction, and receptor channel activation is required. Furthermore, inhibition of phosphatase 2B, but not phosphatase 1 or 2A, prevented GABA(A) receptor inhibition by glycine receptor activation, whereas inhibition of phosphorylation pathways rendered cross-talk irreversible. Taken together, our results demonstrated that there is an asymmetric cross-inhibition between glycine and GABA(A) receptors and that a selective modulation of the state of phosphorylation of GABA(A) receptor and/or mediator proteins underlies the asymmetry in the cross-inhibition.
Indexed Type: SCI
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2000
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Li-2003-Asymmetric cross-inh.pdf(330KB)----开放获取View Download

Recommended Citation:
Li, Y; Wu, LJ; Legendre, P; Xu, TL.Asymmetric cross-inhibition between GABA(A) and glycine receptors in rat spinal dorsal horn neurons,JOURNAL OF BIOLOGICAL CHEMISTRY,2003,278(40):38637-38645
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Y]'s Articles
[Wu, LJ]'s Articles
[Legendre, P]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Y]‘s Articles
[Wu, LJ]‘s Articles
[Legendre, P]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Li-2003-Asymmetric cross-inh.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!