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Title: A simple and effective method for obtaining stable in vivo whole-cell recordings from visual cortical neurons
Author: Zhu, Z ; Wang, Y ; Xu, XZ ; null(李澄宇)
Source: CEREBRAL CORTEX
Issued Date: 2002
Volume: 12, Issue:6, Pages:585-589
Keyword: CORTEX
Subject: Neurosciences & Neurology
Corresponding Author: Li, CY (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,
English Abstract: We describe a simple and effective method for obtaining stable in vivo whole-cell recordings in cat visual cortex. The core of the new approach is to prevent brain pulsation by retaining the dura mater. After being treated with an enzyme (collagenase), the dura became soft enough to allow easy penetration by a patch-clamp electrode with negligible damage to the tip. The procedure is as simple as those used for extracellular recordings, and all the intricate steps required for conventional techniques are no longer necessary. The reliability of this approach is demonstrated by stable and sustained intracellular recordings and high-quality intracellular staining. The method is especially effective for studying small neurons in the superficial layers immediately below the dura.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2058
Appears in Collections:神经所(总)_期刊论文

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Zhu, Z; Wang, Y; Xu, XZ; Li, CY.A simple and effective method for obtaining stable in vivo whole-cell recordings from visual cortical neurons,CEREBRAL CORTEX,2002,12(6):585-589
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