中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Regulation of neuromuscular synapse development by glial cell line-derived neurotrophic factor and neurturin
Author: Wang, CY ; Yang, F ; He, XP ; Je, HS ; Zhou, JZ ; Eckermann, K ; Kawamura, D ; Feng, L ; Shen, L ; Lu, B
Source: JOURNAL OF BIOLOGICAL CHEMISTRY
Issued Date: 2002
Volume: 277, Issue:12, Pages:10614-10625
Keyword: NERVE-MUSCLE INTERACTIONS ; LONG-TERM POTENTIATION ; GDNF-FAMILY ; MOTOR-NEURONS ; DEVELOPING MOTONEURONS ; HIPPOCAMPAL-NEURONS ; TRANSMITTER RELEASE ; QUANTAL SECRETION ; CORTICAL-NEURONS ; VISUAL-CORTEX
Subject: Biochemistry & Molecular Biology
Corresponding Author: Lu, B (reprint author), NICHHD, NIH, Unit Synapse Dev & Plast, Bldg 49,Rm 6A-80,49 Convent Dr,MSC4480, Washington, DC 20052 USA,
English Abstract: Glial cell line-derived neurotrophic factor (GDNF) is known for its potent effect on neuronal survival, but its role in the development and function of synapses is not well studied. Using Xenopus nerve-muscle co-cultures, we show that GDNF and its family member neurturin (NRTN) facilitate the development of the neuromuscular junction (NMJ). Long-term application of GDNF significantly increased the total length of neurites in the motoncurons. GDNF also caused an increase in the number and the size of synaptic vesicle clustering, as demonstrated by synaptobrevin-GFP fluorescent imaging, and FM dye staining. Electrophysiological experiments revealed two effects of GDNF on synaptic transmission at NMJ. First, GDN-F markedly increased the frequency of spontaneous transmission and decreased the variability of evoked transmission, suggesting an enhancement of transmitter secretion. Second, GDNF elicited a small increase in the quantal size, without affecting the average rise and decay times of synaptic currents. Imaging analysis showed that the size of acetylcholine receptor clusters at synapses increased in muscle cells overexpressing GDNF. Neurturin had very similar effects as GDNF. These results suggest that GDNF and NRTN are new neuromodulators that regulate the development of the neuromuscular synapse through both pre- and postsynaptic mechanisms.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2064
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
J. Biol. Chem.-2002-Wang-10614-25.pdf(811KB)----开放获取View Download

Recommended Citation:
Wang, CY; Yang, F; He, XP; Je, HS; Zhou, JZ; Eckermann, K; Kawamura, D; Feng, L; Shen, L; Lu, B.Regulation of neuromuscular synapse development by glial cell line-derived neurotrophic factor and neurturin,JOURNAL OF BIOLOGICAL CHEMISTRY,2002,277(12):10614-10625
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, CY]'s Articles
[Yang, F]'s Articles
[He, XP]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, CY]‘s Articles
[Yang, F]‘s Articles
[He, XP]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: J. Biol. Chem.-2002-Wang-10614-25.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!