中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 期刊论文
Title: Activity- and Ca2+-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons
Author: Du, J ; Feng, LY ; Yang, F ; Lu, B
Source: JOURNAL OF CELL BIOLOGY
Issued Date: 2000
Volume: 150, Issue:6, Pages:1423-1433
Keyword: TrkB receptors ; tetanic stimulation ; calcium influx ; Ca2+/calmodulin-dependent kinase II ; synaptic transmission ; NERVE GROWTH-FACTOR ; LONG-TERM POTENTIATION ; CORTICAL DENDRITIC GROWTH ; BDNF KNOCKOUT MICE ; SYNAPTIC TRANSMISSION ; VISUAL-CORTEX ; CREB PHOSPHORYLATION ; GENE-EXPRESSION ; RAT HIPPOCAMPUS ; CA1 REGION
Subject: Cell Biology
Corresponding Author: Lu, B (reprint author), NICHHD, Unit Synapse Dev & Plast, Dev Neurobiol Lab, NIH, Bldg 49,Rom 5A38,49 Convent Dr, Bethesda, MD 20892 USA,
English Abstract: Brain-derived neurotrophic factor (BDNF) has been shown to regulate neuronal survival and synaptic plasticity in the central nervous system (CNS) in an activity-dependent manner, but the underlying mechanisms remain unclear, Here we report that the number of BDNF receptor TrkB on the surface of hippocampal neurons can be enhanced by high frequency neuronal activity and synaptic transmission, and this effect is mediated by Ca2+ influx. Using membrane protein biotinylation as well as receptor binding assays, we show that field electric stimulation increased the number of TrkB on the surface of cultured hippocampal neurons. Immunofluorescence staining suggests that the electric stimulation facilitated the movement of TrkB from intracellular pool to the cell surface, particularly on neuronal processes. The number of surface TrkB was regulated only by high frequency tetanic stimulation, but not by low frequency stimulation. The activity de pendent modulation appears to require Ca2+ influx, since treatment of the neurons with blockers of voltage-gated Ca-2+ channels or NMDA receptors, or removal of extracellular Ca2+, severely attenuated the effect of electric stimulation. Moreover, inhibition of Ca2+/calmodulin-dependent kinase II (CaMKII) significantly reduced the effectiveness of the tetanic stimulation. These findings may help us to understand the role of neuronal activity in neurotrophin function and the mechanism for receptor tyrosine kinase signaling.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2085
Appears in Collections:神经所(总)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Du-2000-Activity- and Ca2+-d.pdf(541KB)----开放获取View Download

Recommended Citation:
Du, J; Feng, LY; Yang, F; Lu, B.Activity- and Ca2+-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons,JOURNAL OF CELL BIOLOGY,2000,150(6):1423-1433
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Du, J]'s Articles
[Feng, LY]'s Articles
[Yang, F]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Du, J]‘s Articles
[Feng, LY]‘s Articles
[Yang, F]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Du-2000-Activity- and Ca2+-d.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!