中国科学院上海生命科学研究院神经科学研究所机构知识库
Advanced  
SIBS OpenIR  > 神经所(总)  > 学位论文
Title: 1、小鼠视网膜神经节细胞形态学分类的研究2、家兔视网膜方向选择性神经节细胞的生理和药理特性和视动震颤关系的研究
Author: 孙文智
Degree Level: 博士
Issued Date: 2004
Degree Grantor: 中国科学院神经科学研究所
Place of Degree Grantor: 中国科学院神经科学研究所
Supervisor: 何士刚
Keyword: 形态学 ; 分枝模式 ; 分枝层次 ; 基因枪 ; 视网膜方向选择性神经节细胞 ; 视动震颤 ; 全细胞 ; 电生理 ; 药理学 ; 家兔
Alternative Title: English Abstract Morphological Classification of Mouse Retinal Ganglion Cells
Abstract: 我们用“DiOListic”方法标记小鼠视网膜的铺片标本,收集了有520个神经节细胞进行照相和测量,并根据它们的形态学特性进行了分类。用基因枪把包被有脂溶性染料DII的钨颗粒发射到视网膜的铺片标本中,当这些颗粒接触到的细胞膜时细胞的形态就会被完整地显示出来。在实验中,我们测量和计算了这些神经节细胞的胞体大小、树突野大小和树突在IPL中分枝层次。根据测量结果以及细胞树突的分枝模式,我们把520个神经节细胞被分为四个大类。其中树突分枝是单层有三个大类:有大细胞体和大树突野的A类、有相对较小细胞体和树突野的B类以及.较小细胞体和相对较大树突野的c类细胞。树突丛分是双层的细胞被定义为D类。这项研究是迄今为止对小鼠视网膜神经节细胞的形态学最为详尽的研究,也为进一步研究基因改变的小鼠视网膜的形态学性质和生理功能提供了一个基础。视动震颤(OKN)是一类眼球运动,被认为能减少视网膜图像运动从而实现稳定视网膜图像的功能。现有的证据表明视网膜方向选择性神经节细胞的输出可能参一与了驭动视动震颤。在本实验中我们用电生理记录和行为观察相结合的方法来探讨视网膜方向选择性神经节细胞的输出反应和OKN的相关性。许多药理学研究结果证明某些药物能消除视网膜方向选择性,或改变视网膜方向选择性神经节细胞的输出。我们在眼内注射这些药物并观察到了它们对OKN的影响。我们估算了眼内注射的药物的浓度,并用全细胞膜片钳记录了视网膜方向选择性神经节细胞对相同浓度药物的反应。眼内注射NMDA受体的阻断剂AP-7不影响眼动,AP-7对ON和ON-OFF型视网膜方向选择性神经节细胞具有相同的效果,即将两种细胞对光反应削弱大约30%。眼内注射n型ACh受体的拮抗剂D-TC可以完全阻断OKN眼动,电生理记录显示D-TC将ON型视网膜方向选择性神经节细胞对方向性运动刺激反应削弱大约70%,而只将ON-OFF型视网膜方向选择性神经节细胞反应削弱大约30%。眼内注射20μM的NMDA能诱发震颤,相同浓度的NMDA能使ON型视网膜方向选择细胞产生很强的放电反应,而ON-OFF型的则不能。ON型视网膜方向选择J目荆,经节细胞的NMDA浓度一反应曲线和NMDA驱动的眼动的浓度一反应曲线有极好的吻合,而ON-OFF型的NMDA浓度一反应曲线则与眼动不相、关。这些结果提示ON型视网膜方向选择性神经节细胞的活动对驱动OKN眼动即使是充分的,也是必要的。
English Abstract: Five hundred twenty ganglion cells in an isolated whole-mount preparation of the mouse retina were labeled using the "DiOlistic" method and were classified according to their morphological properties. Tungsten particles coated with a lipophilic dye (Dil) were propelled into the whole-mount retina using a gene gun. When a dye-coated particle contacted the cell membrane, the entire cell was labeled. The ganglion cells were classified into four groups based on their soma size, dendritic field size, and pattern and level of stratification. Broadly monostratified cells were classified into three groups: RGA cells (large soma, large dendritic field), RGB cells (small to medium-sized soma, small to medium-sized dendritic field), and RGc cells (small to medium-sized size soma, medium-sized to large dendritic field). Bistratified cells were classified as RGD. This study represents the most complete morphological classification of mouse retinal ganglion cells available to date and provides a foundation for further understanding of the correlation of physiology and morphology and ganglion cell function with genetically manipulated animals. Optokinetic Nystagmus (OKN) is a type of eye movement thought to minimize retinal image motion. Existing evidence indicate that both types of direction selective ganglion cells (DSGCs) might involve in driving OKN. In this study we attempted to correlate results of behavioral studies with responses of electrophysiological recorded DSGCs. Various reagents known to affect retinal DS were intraocularly injected and the impacts on OKN observed. Intraocular concentration was. estimated for each reagent and used in vitro experiments observing electrophysiological responses of DSGCs to these reagents recorded in whole cell mode. NMDA antagonist AP-7 suppressed responses to a drifting bar of both DSGCs by about 30%, but did not affect the OKN. Intraocular injection of nicotinic ACh receptor antagonist D-tubocurarine completely blocked OKN, and suppressed the responses of ON DSGCs to a drifting bar by 70%, but suppressed the ON-OFF DSGCs' response only by 30%, Intraocular injection of 20 uMNMDA induced nystagmus, same concentration elicits vigorous spiking in ON DSGCs but never on ON-OFF DSGCs. The dose-response curve of NMDA induced nystagmus corresponded to that of the spiking response of the ONDSGCs. These results suggested that activation of the ON DSGCs is sufficient and necessary in driving nystagmus.
Language: 中文
Content Type: 学位论文
URI: http://ir.sibs.ac.cn/handle/331001/2252
Appears in Collections:神经所(总)_学位论文

Files in This Item:
File Name/ File Size Content Type Version Access License
LW013861.pdf(2094KB)----暂不开放 联系获取全文

Recommended Citation:
1、小鼠视网膜神经节细胞形态学分类的研究2、家兔视网膜方向选择性神经节细胞的生理和药理特性和视动震颤关系的研究.孙文智[d].中国科学院神经科学研究所,2004.20-25
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[孙文智]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[孙文智]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!