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Title: Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells
Author: Wang, D ; She, L ; Sui, YN ; Yuan, XB ; Wen, YQ ; null(蒲慕明)
Source: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Issued Date: 2012
Volume: 109, Issue:51, Pages:E3558-E3567
Keyword: NEURONAL GROWTH CONES ; F-ACTIN FLOW ; CYTOSKELETAL DYNAMICS ; CULTURED HIPPOCAMPAL ; LATERAL DIFFUSION ; CORTICAL FLOW ; NERVE GROWTH ; ANIMAL-CELLS ; FIBROBLASTS ; SURFACE
Subject: Science & Technology - Other Topics
Corresponding Author: Poo, MM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China.,mpoo@berkeley.edu
English Abstract: Directional flow of membrane components has been detected at the leading front of fibroblasts and the growth cone of neuronal processes, but whether there exists global directional flow of plasma membrane components over the entire migrating neuron remains largely unknown. By analyzing the trajectories of antibody-coated single quantum dots (QDs) bound to two membrane proteins, overexpressed myc-tagged synaptic vesicle-associated membrane protein VAMP2 and endogenous neurotrophin receptor TrkB, we found that these two proteins exhibited net forward transport, which is superimposed upon Brownian motion, in both leading and trailing processes of migrating cerebellar granule cells in culture. Furthermore, no net directional transport of membrane proteins was observed in nonmigrating cells with either growing or stalling leading processes. Analysis of the correlation of motion direction between two QDs on the same process in migrating neurons also showed a higher frequency of correlated forward than rearward movements. Such correlated QD movements were markedly reduced in the presence of myosin II inhibitor blebbistatin, suggesting the involvement of myosin II-dependent active transport processes. Thus, a net forward transport of plasma membrane proteins exists in the leading and trailing processes of migrating neurons, in line with the translocation of the soma.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2482
Appears in Collections:神经所(总)_期刊论文

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Wang, D; She, L; Sui, YN; Yuan, XB; Wen, YQ; Poo, MM.Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells,PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(51):E3558-E3567
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