Ding, XY (reprint author), Chinese Acad Sci, Grad Sch, State Key Lab Cell Biol, Inst Biochem & Cell Biol,SIBS, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,email@example.com
P-4-ATPases are lipid flippases that transport phospholipids across cellular membranes, playing vital roles in cell function. In humans, the disruption of the P-4-ATPase ATP8A2 gene causes a severe neurological phenotype. Here, we found that Atp8a2 mRNA was highly expressed in PC12 cells, hippocampal neurons and the brain. Overexpression of ATP8A2 increased the length of neurite outgrowth in NGF-induced PC12 cells and in primary cultures of rat hippocampal neurons. Inducing the loss of function of CDC50A in hippocampal neurons via RNA interference reduced neurite outgrowth, and the co-overexpression of CDC50A and ATP8A2 in PC12 cells enhanced NGF-induced neurite outgrowth. These results indicate that ATP8A2, acting in synergy with CDC50A, performs an important role in neurite outgrowth in neurons. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.