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Title: The Effects of Cobalt on the Development, Oxidative Stress, and Apoptosis in Zebrafish Embryos
Author: Cai, GQ ; Zhu, JF ; Shen, C ; Cui, YM ; null(杜久林) ; Chen, XD
Source: BIOLOGICAL TRACE ELEMENT RESEARCH
Issued Date: 2012
Volume: 150, Issue:1, Pages:200-207
Keyword: IN-VITRO FERTILIZATION ; TOTAL HIP-ARTHROPLASTY ; ANTIOXIDANT ENZYMES ; FOLLICULAR-FLUID ; BEARING ; CELLS ; MICE ; SUPPLEMENTATION ; CASPASE-3 ; MELATONIN
Subject: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
Corresponding Author: Chen, XD (reprint author), Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Orthopaed, 1665 Kong Jiang Rd, Shanghai 200092, Peoples R China.,chenxdmd@163.com
English Abstract: Metal-on-metal hip arthroplasty has been performed with increasing frequency throughout the world, particularly in younger and more active patients, including women of childbearing age. The potential toxicity of cobalt exposure on fetus is concerned since cobalt ions generated by metal-on-metal bearings can traverse the placenta and be detected in fetal blood and amniotic fluid. This study examined the effects of cobalt exposure on early embryonic development and the mechanisms underlying its toxicity. Zebrafish embryos were exposed to a range of cobalt concentrations (0-100 mg/L) between 1 and 144 h postfertilization. The survival and early development of embryos were not significantly affected by cobalt at concentrations < 100 mu g/L. However, embryos exposed to higher concentrations (> 100 mu g/L) displayed reduced survival rates and abnormal development, including delayed hatching, aberrant morphology, retarded growth, and bradycardia. Furthermore, this study examined oxidative stress and apoptosis in embryos exposed to cobalt at concentrations of 0-500 mu g/L. Lipid peroxidation levels were increased in cobalt-treated embryos at concentrations of 100 and 500 mu g/L. The mRNA levels of catalase, superoxide dismutase 2, p53, caspase-3, and caspase-9 genes were upregulated in a dose-dependent manner. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assays also revealed abnormal apoptotic signals in the brain, trunk, and tail when treated with 500 mu g/L cobalt. These data suggest that oxidative stress and apoptosis are associated with cobalt toxicity in zebrafish embryos.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2503
Appears in Collections:神经所(总)_期刊论文

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Cai, GQ; Zhu, JF; Shen, C; Cui, YM; Du, JL; Chen, XD.The Effects of Cobalt on the Development, Oxidative Stress, and Apoptosis in Zebrafish Embryos,BIOLOGICAL TRACE ELEMENT RESEARCH,2012,150(1):200-207
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