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Title: A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
Author: Lukinavicius, G ; Umezawa, K ; Olivier, N ; Honigmann, A ; Yang, GY ; Plass, T ; Mueller, V ; Reymond, L ; Correa, IR ; Luo, ZG ; Schultz, C ; Lemke, EA ; Heppenstall, P ; Eggeling, C ; Manley, S ; Johnsson, K
Source: NATURE CHEMISTRY
Issued Date: 2013
Volume: 5, Issue:2, Pages:132-139
Keyword: DIELS-ALDER REACTIONS ; LIVING CELLS ; FUSION PROTEINS ; FAR-RED ; FLUORESCENCE MICROSCOPY ; SYNTHETIC FLUOROPHORES ; STIMULATED-EMISSION ; STED NANOSCOPY ; SI-RHODAMINE ; MOUSE-BRAIN
Subject: Chemistry
Corresponding Author: Lukinavicius, G (reprint author), Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn ISIC, Inst Bioengn, Natl Ctr Competence Res NCCR Chem Biol, CH-1015 Lausanne, Switzerland.,kai.johnsson@epfl.ch
English Abstract: The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.
Indexed Type: sci
Language: 英语
Content Type: 期刊论文
URI: http://ir.sibs.ac.cn/handle/331001/2512
Appears in Collections:神经所(总)_期刊论文

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Recommended Citation:
Lukinavicius, G; Umezawa, K; Olivier, N; Honigmann, A; Yang, GY; Plass, T; Mueller, V; Reymond, L; Correa, IR; Luo, ZG; Schultz, C; Lemke, EA; Heppenstall, P; Eggeling, C; Manley, S; Johnsson, K.A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins,NATURE CHEMISTRY,2013,5(2):132-139
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